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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
12/09/2014 |
Data da última atualização: |
29/04/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MÜLLER, B. S. de F.; SAKAMOTO, T.; SILVEIRA, R. D. D.; ZAMBUSSI-CARVALHO, P. F.; PEREIRA, M.; PAPPAS JUNIOR, G. J.; COSTA, M. M. do C.; GUIMARÃES, C. M.; PEREIRA, W. J.; BRONDANI, C.; VIANELLO-BRONDANI, R. P. |
Afiliação: |
BÁRBARA SALOMÃO DE FARIA MÜLLER, BIOAGRO - UFV; TETSU SAKAMOTO, UFMG; RICARDO DIÓGENES DIAS SILVEIRA; PATRICIA FERNANDA ZAMBUSSI-CARVALHO, UFG; MARISTELA PEREIRA, UFG; GEORGIOS JOANIS PAPPAS JUNIOR, UNB; MARCOS MOTA DO CARMO COSTA, CENARGEN; CLEBER MORAIS GUIMARAES, CNPAF; WENDELL JACINTO PEREIRA; CLAUDIO BRONDANI, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
Título: |
Differentially expressed genes during flowering and grain filling in common bean (Phaseolus vulgaris) grown under drought stress conditions. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Plant Molecular Biology Reporter, Athens, v. 32, p. 438-451, 2014. |
DOI: |
10.1007/s11105-013-0651-7 |
Idioma: |
Inglês |
Conteúdo: |
Drought stress, particularly during the flowering and grain-filling stages of growth, contributes to serious yield loss in common bean (Phaseolus vulgaris L.). The aim of this study was to identify genes induced in response to drought stress using transcriptome analysis of contrasting genotypes. Using leaf tissues of tolerant (BAT 477) and susceptible common bean genotypes (Pérola), collected at the flowering and grain-filling stages, four complementary deoxyribonucleic acid representational difference analysis subtractive libraries were constructed and then sequenced. A total of 7,203 (77.6 %) sequences with an average sequence size of 570 bp were considered valid, for a combined 4 Mbp sequence. According to a differential display analysis, 802 expressed sequence tags, distributed across 67 contigs, were differentially expressed by the tolerant (37 contigs) and susceptible genotypes (30 contigs) after identification under drought conditions during the two investigated plant developmental stages. Of these differential contigs, the 13 most frequent genes were exclusive to the tolerant genotype. Based on BLAST2GO, 73 % of the gene sequences were annotated and 12 % showed mapping results, with the highest similarity rate corresponding to Glycine max (41 %). According to gene ontology functional analysis, 48 % of the sequences were attributed to cell metabolic processes. Overall, 8.3 % of the transcribed sequences exhibited similarity to transcription factors, predominantly those of the AP2-EREBP family (97.8 %). Of the target sequences validated by quantitative real-time polymerase chain reaction, most genes showed an expression level that agreed with that predicted by in silico analysis. Thus, the drought transcriptome dataset is a valuable resource on the variation in these gene sequences, offering the opportunity to identify robust molecular markers tightly linked to trait-controlling loci for use in marker-assisted breeding. MenosDrought stress, particularly during the flowering and grain-filling stages of growth, contributes to serious yield loss in common bean (Phaseolus vulgaris L.). The aim of this study was to identify genes induced in response to drought stress using transcriptome analysis of contrasting genotypes. Using leaf tissues of tolerant (BAT 477) and susceptible common bean genotypes (Pérola), collected at the flowering and grain-filling stages, four complementary deoxyribonucleic acid representational difference analysis subtractive libraries were constructed and then sequenced. A total of 7,203 (77.6 %) sequences with an average sequence size of 570 bp were considered valid, for a combined 4 Mbp sequence. According to a differential display analysis, 802 expressed sequence tags, distributed across 67 contigs, were differentially expressed by the tolerant (37 contigs) and susceptible genotypes (30 contigs) after identification under drought conditions during the two investigated plant developmental stages. Of these differential contigs, the 13 most frequent genes were exclusive to the tolerant genotype. Based on BLAST2GO, 73 % of the gene sequences were annotated and 12 % showed mapping results, with the highest similarity rate corresponding to Glycine max (41 %). According to gene ontology functional analysis, 48 % of the sequences were attributed to cell metabolic processes. Overall, 8.3 % of the transcribed sequences exhibited similarity to transcription factors, predominantly thos... Mostrar Tudo |
Thesagro: |
Deficiência hídrica; Feijão; Phaseolus vulgaris. |
Thesaurus Nal: |
Abiotic stress; Beans; Water stress. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/180004/1/Muller2014-Article-DifferentiallyExpressedGenesDu.pdf
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Marc: |
LEADER 02986naa a2200325 a 4500 001 1994817 005 2024-04-29 008 2014 bl uuuu u00u1 u #d 024 7 $a10.1007/s11105-013-0651-7$2DOI 100 1 $aMÜLLER, B. S. de F. 245 $aDifferentially expressed genes during flowering and grain filling in common bean (Phaseolus vulgaris) grown under drought stress conditions.$h[electronic resource] 260 $c2014 520 $aDrought stress, particularly during the flowering and grain-filling stages of growth, contributes to serious yield loss in common bean (Phaseolus vulgaris L.). The aim of this study was to identify genes induced in response to drought stress using transcriptome analysis of contrasting genotypes. Using leaf tissues of tolerant (BAT 477) and susceptible common bean genotypes (Pérola), collected at the flowering and grain-filling stages, four complementary deoxyribonucleic acid representational difference analysis subtractive libraries were constructed and then sequenced. A total of 7,203 (77.6 %) sequences with an average sequence size of 570 bp were considered valid, for a combined 4 Mbp sequence. According to a differential display analysis, 802 expressed sequence tags, distributed across 67 contigs, were differentially expressed by the tolerant (37 contigs) and susceptible genotypes (30 contigs) after identification under drought conditions during the two investigated plant developmental stages. Of these differential contigs, the 13 most frequent genes were exclusive to the tolerant genotype. Based on BLAST2GO, 73 % of the gene sequences were annotated and 12 % showed mapping results, with the highest similarity rate corresponding to Glycine max (41 %). According to gene ontology functional analysis, 48 % of the sequences were attributed to cell metabolic processes. Overall, 8.3 % of the transcribed sequences exhibited similarity to transcription factors, predominantly those of the AP2-EREBP family (97.8 %). Of the target sequences validated by quantitative real-time polymerase chain reaction, most genes showed an expression level that agreed with that predicted by in silico analysis. Thus, the drought transcriptome dataset is a valuable resource on the variation in these gene sequences, offering the opportunity to identify robust molecular markers tightly linked to trait-controlling loci for use in marker-assisted breeding. 650 $aAbiotic stress 650 $aBeans 650 $aWater stress 650 $aDeficiência hídrica 650 $aFeijão 650 $aPhaseolus vulgaris 700 1 $aSAKAMOTO, T. 700 1 $aSILVEIRA, R. D. D. 700 1 $aZAMBUSSI-CARVALHO, P. F. 700 1 $aPEREIRA, M. 700 1 $aPAPPAS JUNIOR, G. J. 700 1 $aCOSTA, M. M. do C. 700 1 $aGUIMARÃES, C. M. 700 1 $aPEREIRA, W. J. 700 1 $aBRONDANI, C. 700 1 $aVIANELLO-BRONDANI, R. P. 773 $tPlant Molecular Biology Reporter, Athens$gv. 32, p. 438-451, 2014.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registros recuperados : 51 | |
5. | | GRYNBERG, P.; GUIMARÃES, L. A.; COSTA, M. M. do C.; TOGAWA, R. C.; BRASILEIRO, A. C. M.; GUIMARAES, P. M. Comprehensive profiling and characterization of Arachis stenosperma (peanut) and Meloidogyne arenaria (plant-root nematode) small-RNAs identified during the course of the infection. In: INTERNATIONAL CONFERENCE OF THE AB3C, 13., 2017, São Pedro. Proceedings... [S.l.]: AB3C, 2017. p. 170Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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9. | | SALGADO, F. F.; LEAO, A. P.; GRYNBERG, P.; COSTA, M. M. do C.; TOGAWA, R. C.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Prediction and annotation of miRNAs and the preponderant role of transcription factors in the response of Elaeis guineensis Jacq to abiotic stresses of drought and salinity. In: BRAZILIAN SYMPOSIUM ON BIOINFOMATICS (BSB 2023), 16., 2023, Curitiba, PR. [Proceedings...]. Porto Alegre, RS: Sociedade Brasileira de Computação, 2023.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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10. | | GOMES, T. G.; ALVES, G. S. C.; HADI, S. I. I. A.; COSTA, M. M. do C.; MENDONCA, S.; MILLER, R. N. G.; SIQUEIRA, F. G. de. Expressão diferencial de genes em Pleurotus pulmonarius associados a degradação enzimática e destoxificação da torta de pinhão-manso. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 18.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agroenergia. |
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11. | | BERGMANN, J. C.; COSTA, M. M. do C.; JUNIOR, P. G. J.; BARRETO, C. C.; KRUGER, R. H.; QUIRINO, B. F. Soil bacterial community structure associated with oil palm (Elaeis guineensis) fatal yellowing disease. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 36., 2014, Flórida. Abstracts... Fairfax: Society for Industrial Microbiology and Biotechnology, 2014.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agroenergia. |
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12. | | ANDRADE, E. L. P. de; LOPES JUNIOR, S.; COSTA, M. M. do C.; RAMOS, R. G. C.; FARIAS, J. L. S.; SILVA, A. M. D.; PROCOPIO, C. D. Utilização de tecnologia orientada a objetos no desenvolvimento de sistemas de informação corporativos na Embrapa. In: TURAZI, A. (Ed.). O recurso tecnologia da informação em uma instituição de pesquisa agropecuária: de 1974 a 2000. Brasília, DF: Embrapa Comunicação para Transferência de Tecnologia, 2001. p. 195-224.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Arroz e Feijão. |
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13. | | FALCAO, L. L.; WERNECK, J. O. S.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; SILVA JUNIOR, O. B. da; ALVES, R. M.; ALBUQUERQUE, P. S. B.; MARCELLINO, L. H. Analyses of cupuassu (Theobroma grandiflorum) transcriptome during interaction with Moniliophthora perniciosa, the causal agent of Witches' Broom disease. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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14. | | FALCAO, L. L.; WERNECK, J. O. S.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; SILVA JUNIOR, O. B. da; ALVES, R. M.; ALBUQUERQUE, P. S. B.; MARCELLINO, L. H. Analyses of cupuassu (Theobroma grandiflorum) transcriptome during interaction with Moniliophthora perniciosa, the causal agent of Witches' Broom disease. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental. |
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15. | | MIDORIKAWA, G. E. O.; CORREA, C. L.; NORONHA, E. F.; FERREIRA FILHO, E. X.; TOGAWA, R. C.; COSTA, M. M. do C.; SILVA JUNIOR, O. B. da; GRYNBERG, P.; MILLER, R. N. G. Analysis of the transcriptome in Aspergillus tamarii during enzymatic degradation of sugarcane bagasse. Frontiers in Bioengineering and Biotechnology, v 6, article 123, 2018. Na publicação: Orzenil Bonfim Silva-Junior.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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16. | | FALCAO, L. L.; WERNECK, J. O. S.; ALBUQUERQUE, P. S. B.; ALVES, R. M.; GRYNBERG, P.; TOGAWA, R. C.; COSTA, M. M. do C.; BRIGIDO, M. M.; MARCELLINO, L. H. Comparative transcriptomics of cupuassu (Theobroma grandiflorum) offers insights into the early defense mechanism to Moniliophthora perniciosa, the causal agent of witches' broom disease. Journal of Plant Interactions, v. 17, n. 1, p. 991-1005, 2022. Na publicação; Joseilde Oliveira Silva-Werneck.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia. |
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17. | | GRYNBERG, P.; TOGAWA, R. C.; FREITAS, L. D. de; ANTONINO, J. D.; RANCUREL, C.; COSTA, M. M. do C.; GROSSI-DE-SA, M. F.; MILLER, R. N. G.; BRASILEIRO, A. C. M.; GUIMARAES, P. M.; DANCHIN, E. G. J. Comparative genomics reveals novel target genes towards specific control of plant-parasitic nematodes. Genes, v. 11, 1347, 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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18. | | SARTOR, T.; FALAVIGNA, V. da S.; CATTANI, A. M.; SILVEIRA, C. P.; MALABARBA, J.; PORTO, D. D.; GRYNBERG, P.; TOGAWA, R. C.; COSTA, M. M. do C.; SANTOS, H. P. dos; PASQUALI, G.; REVERS, L. F. Caracterização da função do gene FLC-LIKE durante a transição da ENDO- para dormência na macieira. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8, 2023, Florianópolis, SC. Anais...FLorianópolis: SBG, 30 de maio a 2 de junho de 2023. p. 67Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Uva e Vinho. |
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19. | | SARTOR, T.; FALAVIGNA, V. da S.; CATTANI, A. M.; SILVEIRA, C. P.; MALABARBA, J.; PORTO, D. D.; GRYNBERG, P.; TOGAWA, R. C.; COSTA, M. M. do C.; SANTOS, H. P. dos; PASQUALI, G.; REVERS, L. F. Caracterização da função do gene FLC-LIKE durante a transição da Endo- para ecodormencia na macieira. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8, 2023, Florianópolis, SC. Anais...Florianópolis: SBG, 2023. p. 67.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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20. | | MILLER, R. N. G.; PASSOS, M. A. N.; MENEZES, N. N. P.; SOUZA JUNIOR, M. T.; COSTA, M. M. do C.; AZEVEDO, V. C. R.; AMORIM, E. P.; PAPPAS JUNIOR, G. J.; CIAMPI, A. Y. Characterization of novel microsatellite markers in Musa acuminata subsp. burmannicoides, var. Calcutta 4. BMC Research Notes, v.3, n.148, 2010. Disponível em:.Acesso em: 16 ago. 2010.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 51 | |
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